Paratrichodorus divergens sp. n., a new potential virus vector of tobacco rattle virus and additional observations on P. hispanus Roca & Arias, 1986 from Portugal (Nematoda: Trichodoridae)
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منابع مشابه
Eliminating Tobacco Rattle Virus from Viruliferous Paratrichodorus allius and Establishing a New Virus-Vector Combination.
A reliable method to eliminate tobacco rattle virus (TRV) from viruliferous Paratrichodorus allius populations was developed. This virus is vectored by P. allius in the Pacific Northwest and causes corky ringspot disease (CRS) of potato. The viruliferous nematodes that were reared on 'Vernema' alfalfa or '770' scotch spearmint for at least 3 months did not transmit TRV to 'Samsun NN' tobacco, a...
متن کاملImmunogold Localization of Tobacco Rattle Virus Particles within Paratrichodorus anemones.
Unequivocal evidence of the viral nature of virus-like particles observed at the specific site of retention of tobacco rattle virus (TRV) in Paratrichodorus and Trichodorus nematodes has not previously been available. A new staining technique using safranin-O, which does not affect viral antigenicity, was used with an antiserum raised against the coat protein of TRV and prepared for use with im...
متن کاملEfficient virus-induced gene silencing in roots using a modified tobacco rattle virus vector.
Due to their capability of eliciting a form of posttranscriptional gene silencing (termed virus-induced gene silencing or VIGS), plant viruses are increasingly used as reverse-genetics tools for functional characterization of plant genes. RNA viruses have been shown to trigger silencing in a variety of host plants, including members of Solanacae and Arabidopsis (Arabidopsis thaliana). Several f...
متن کاملBreakthrough Technologies Efficient Virus-Induced Gene Silencing in Roots Using a Modified Tobacco Rattle Virus Vector
Due to their capability of eliciting a form of posttranscriptional gene silencing (termed virus-induced gene silencing or VIGS), plant viruses are increasingly used as reverse-genetics tools for functional characterization of plant genes. RNA viruses have been shown to trigger silencing in a variety of host plants, including members of Solanacae and Arabidopsis (Arabidopsis thaliana). Several f...
متن کاملAn antiviral RISC isolated from Tobacco rattle virus-infected plants.
The RNAi model predicts that during antiviral defense a RNA-induced silencing complex (RISC) is programmed with viral short-interfering RNAs (siRNAs) to target the cognate viral RNA for degradation. We show that infection of Nicotiana benthamiana with Tobacco rattle virus (TRV) activates an antiviral nuclease that specifically cleaves TRV RNA in vitro. In agreement with known RISC properties, t...
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ژورنال
عنوان ژورنال: Nematology
سال: 2005
ISSN: 1388-5545,1568-5411
DOI: 10.1163/156854105774355572